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KMID : 0644120130260040066
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Journal of Oriental Gynecology
2013 Volume.26 No. 4 p.66 ~ p.81
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Anti-Inflammatory Effect of Ligustri Lucidi Fructus Water Extract in RAW 264.7 Cells Induced by LPS
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Lee Yong-Hyun
Lim Eun-Mee
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Abstract
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Purpose: This study was carried out to investigate the anti-inflammatory effects of Ligustri Lucidi Fructus water extract (LF) in the lipopolysaccharide (LPS)-induced mouse macrophages RAW 264.7 cell.
Methods: Ligustri Lucidi Fructus was extracted with distilled water (2,000 ml) for 2 hours. In order to evaluate cytotoxicity of LF, 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) assay was performed. To investigate anti-inflammatory effects of LF, the concentration of nitric oxide (NO) was measured with NO assay, cytokine was measured by Bio-Plex cytokine assay, and intracellular calcium (Ca) was measured with Fluo-4 Ca assay in RAW 264.7 cell. And when p-value is below 0.05, it is judged to have the significant difference statistically (P<0.05).
Results:
1. LF showed no cytotoxicity.
2. LF inhibited significantly the production of NO at the concentration of 25, 50 and 100 μg/ml.
3. LF inhibited significantly the production of interleukin (IL)-4, macrophage inflammatory protein (MIP)-1α, granulocyte colony stimulating factor (G-CSF) at the concentration of 25, 50, 100 and 200 μg/ml.
4. LF inhibited significantly the production of granulocyte macrophage-colony stimulating factor (GM-CSF), vascular endothelial growth factor (VEGF) at the concentration
of 50, 100 and 200 μg/ml, the interferon (IFN)-γ at 25, 50 and 100 μg/ml respectively.
5. LF inhibited significantly the production of IL-1β at the concentration of 50
and 200 μg/ml, the IL-5 at 25 and 100 μg/ml, the IL-12p70, MIP-1β at 50 and 100 μg/ml, the regulated on activation, normal T cell expressed and secrete d (RANTES) at 100 and 200 μg/ml respectively.
6. LF inhibited significantly the production of IL-10, interferon gamma-induced protein (IP)-10 at the concentration of 200 μg/ml.
7. LF inhibited significantly the production of intracellular Ca at the concentration of 25, 50, 100 and 200 μg/ml.
Conclusions: These results suggest that LF has anti-inflammatory effect and immuno-modulating activity.
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KEYWORD
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Ligustri Lucidi Fructus, NO, Cytokine, Ca, Anti-inflammation
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